Product Name :
Mouse anti Human Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6), conjugated with FITC
Description :
| Clone 1 | Isotype IgG1 | Product Type Monoclonal Antibody | Units 100 µg | Host Mouse | Species Reactivity Human | Application Flow Cytometry Western Blotting
Background :
Hybridoma produced by the fusion of splenocytes from mice immunized with a unique synthetic peptide derived from the C-terminal region of the EDG6 (S1P4) protein and mouse myeloma cells.
Source :
EDG6 (S1P4) belongs to a family of G-protein coupled receptors whose ligands are lysophospholipids. The ligand for EDG6 (S1P4) is sphingosine-1-phosphate. There are 8 known members of the EDG receptor family and they are implicated in mediating growth related effects such as induction of cellular proliferation, alterations in differentiation and survival and suppression of apoptosis. They also evoke cellular effector functions that are dependent on cytoskeletal responses such as contraction, secretion, adhesion and chemotaxis. EDG receptors are developmentally regulated and differ in tissue distribution. They couple to multiple types of G proteins to signal through ras and MAP kinase, rho, phospholipase C and several protein tyrosine kinases. EDG6 (S1P4) is expressed in lymphoid and hematopoietic tissue and in the lungs. Recently, the designation of the EDG receptors has been modified to include the ligand for the receptor. Thus, the new designation for the EDG6 (S1P4) receptor is the sphingosine 1-phosphate receptor-4 (S1P4). Synonyms: Endothelial cell differentiation gene 6 C-terminal; Sphingosine-1-Phosphate Receptor-4 (S1P4) <
Product :
Product Form: FITC conjugate Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide Purification Method: Protein A/G Chromatography Concentration: See vial for concentration
Specificity :
Applications :
The X1626M antibody can be used for flow cytometry (use 1 µg of antibody per 106 cells). NOTE: Epitope for the antibody is the C-terminal region of the EDG6 protein. The C-terminus is an intracellular region and cells need to be permeablized prior to staining with antibody. Optimal concentration should be evaluated by serial dilutions. Functional Analysis: Flow cytometry staining
Storage :
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles Product Stability: See expiration date on vial Shipping Conditions: Ship at ambient temperature
Caution :
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.
References :
1. Yamazaki, Y., et al. ‘EDG-6 as a putative sphingosine 1-phosphate receptor coupling to Ca(2+) signaling pathway.’ Biochem. Biophys. Res. Commun. 2000, 268, 583-589. 2. Van Brocklyn, J.R., et al. ‘Sphingosine 1-phosphate is a ligand for the G protein-coupled receptor EDG-6.’ Blood 2000, 95, 2624-2629. 3. Takuwa, Y., et al. ‘Subtype-specific, differential activities of the EDG family receptors for sphingosine 1-phosphate, a novel lysophospholipid mediator.’ Mol. Cell Endocrinol. 2001, 177, 3-11. 4. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459
Related websites: https://www.medchemexpress.com/antibodies.html
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